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Identification in silico of putative damage responsive elements (DRE) in promoter regions of the yeast genome Genet. Mol. Biol.
Silva,Walkiria Luckwu de Santana; Cavalcanti,Andre Ricardo de Oliveira; Guimarães,Katia Silva; Morais Jr.,Marcos Antonio de.
We report an in silico analysis to identify nucleotide sequence motifs in DNA repair genes that may define a binding site for regulatory proteins during the induction of those genes by mutagens. The damage responsive elements (DRE) weight matrix generated in this analysis was used to search for homologous sequences in the promoter region of all genes, including putative gene and hypothetical open reading frames (ORFs), in the Saccharomyces Genome Data Base (SGD). The results demonstrated that over one third of the yeast genes in the database presented at least one 15-bp sequence in their promoter region with 85% or more of similarity to the DRE consensus sequence. The presence of the DRE sequence in the promoter region of regulatory genes and its high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA binding site; DNA repair; Gene promoter; Gene regulation; Transfac; Weight matrix.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500025
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